Thursday, 25 April 2013

Install Burrows-Wheeler Aligner (BWA) in Linux

Steps to install BWA in linux machine

1. Download BWA from http://bio-bwa.sourceforge.net/
2. Save bwa-0.7.4.tar.bz2 it to your drive (eg: /usr/username)
3. Open the terminal from /usr/username and untar using the command  tar -xvf  bwa-0.7.4.tar.bz2
4. bwa-0.7.4 folder will be created in /usr/username.
5. Change directory to bwa-0.7.4 using command cd  bwa-0.7.4
6. Type command make
7. Create a file bwa.sh in etc/profile.d
8. Open the file and type
    #!/bin/bash
    BWA_HOME=/usr/username/bwa-0.7.3a
    PATH=$BWA_HOME:$PATH
    export PATH

9. Save and close the bwa.sh
10. Open terminal from etc/profile.d
11. Type command
    chmod 755 bwa.sh
12. To check the installation , open the terminal and type bwa.